ADVI MOUNTING MEDIA
Developed in collaboration with the Institute of Molecular Genetics of the Czech Academy of Sciences.
In fluorescence microscopy, the choice of mounting media can make or break the quality of your images. A carefully selected medium stabilizes fluorescent signals, preserves the integrity of delicate structures, and ensures long-term protection, all while maintaining the optical clarity needed for high-resolution imaging. An unsuitable medium can lead to signal fading, structural damage, or compromised results, affecting the reliability of your experiment.
Now, you’re looking at mounting media developed in collaboration with the Institute of Molecular Genetics (IMG) of the Czech Academy of Sciences. These media have been tested in real-world research, designed to provide unmatched fluorescence stability and protection for even the most sensitive biological samples. With these formulations, you can be confident in achieving consistent, high-quality results, no matter the microscopy technique.
When selecting mounting media, you can opt for a universal hardening medium like AD-MOUNT H, ideal for those who prioritize easy and fast handling without specific requirements for preserving volumetric structure. Hardening media also effectively stabilize non-covalently bound dyes, such as Phalloidin. For more delicate applications, a non-hardening medium like AD-MOUNT F offers gentle mounting, ensuring precise preservation of biological volume and preventing cell flattening.
If your focus is on outstanding stabilization of synthetic fluorophores and preventing photobleaching, AD-MOUNT S provides anti-fade properties, though it may not be optimal for protein expression studies. For advanced super-resolution techniques, AD-MOUNT C is the perfect choice, offering a glass-like refractive index, exceptional stabilizing effects, and easy-clearing properties, tailored for the most precise imaging requirements.
For non-hardening mounting media, we recommend using mounting spacers
For non-hardening mounting media, the use of mounting spacers is crucial to maintaining the natural shape and volume of biological samples. Spacers create a controlled gap between the cover slip and the sample, preventing unwanted flattening and preserving delicate structures. This ensures that soft tissues and cellular formations remain intact, providing optimal clarity during imaging. By using spacers in combination with non-hardening media, you can achieve more accurate and reliable results in even the most advanced microscopy techniques.
Universal Application
Refractive index: 1.45
Non-hardening
All fluorophores
Universal mounting medium accommodates a wide-range of fluorophore types. It offers optimized photo-bleaching and fluorescence fading, effectively preserving an integrity and a shape of biological structures.
Designed for long storage of samples.
Superior Stability
Refractive index: 1.47
Non-hardening
Synthetic dyes
Superior Stability mounting medium ensures robust stabilization of fluorescence signal, minimizes photo bleaching in all commonly used synthetic dyes. Preserves the integrity and shape of biological structures.
Designed for long storage of samples.
Clear view
Refractive index: 1.518
Non-hardening
Synthetic dyes
Specifically formulated for high- and super-resolution microscopy. High refractive index, exceptional clearing capabilities, and fluorescence signal stabilization effect. Suitable for commonly used synthetic dyes, but not suitable for expressions and SiR.
Universal Application
Refractive index: 1.45
Hardening
All fluorophores
Universal hardening mounting medium for all fluorophore types. It offers long term stabilization of structures (like phalloidin-stained actin), dyes, minimizing photo-bleaching and fluorescence fading.
Designed for long storage of samples.
A proper selection of mounting media is crucial for successful microscopy experiments. Often, it’s a highly critical step, and the wrong choice can lead to experiment failure. Therefore, we offer three types of mounting media, ranging from very universal to specialized usage.
We recommend to use AD-MOUNT with AD-SEALS.
For assistance in making your selection, please read our brochure or contact us directly.
We would be happy to guide you and share our expertise.
In ADVI-EDU project, we also prepared serial of protocols and video protocols to guide the general sample preparation in microscopy.
VIDEO PROTOCOLS: From Washing to Mounting