The procedure for transferring the cover glass from the petri dish to a drop of PBS on a Parafilm surface involves several careful steps.
Protocol
First, secure a piece of Parafilm on your bench surface using water. Then, use tweezers to carefully lift and remove the cover glass from the petri dish. Swiftly rinse the cover glass with water, gently blotting off any excess to avoid disturbing the adhered cells. Finally, place the cover glass (cell-side down) onto a drop of PBS positioned on the Parafilm. Working on a drop of PBS on Parafilm presents several advantages that facilitate lab procedures. The use of droplets allows for the handling of smaller volumes of solutions, such as antibodies, making the procedure more cost-effective. Moreover, the non-wetting properties of Parafilm ensure easy movement and handling of the droplets without any unwanted spreading. Finally, as Parafilm provides a clean surface, it helps to minimize the potential for contamination, thus improving the reliability of your experimental results.
