Welcome to our page for video protocols, notes, and discussion. Our goal at ADVI-EDU is to make learning about microscopy easy.
With our visual, step-by-step guides, anyone can enhance their skills in this field, no matter where they are in the world.
This video protocol guides you through the process of using AD-SEAL mounting spacers to finalize your microscopic samples. The use of mounting spacers offers several advantages for your microscopic sample preparation. They provide a secure and stable means to attach the cover glass, ensuring that your sample stays in place during imaging. This eliminates the […]
Mounting spacers – How to easy use Read More »
Mounting spacers: ANTI – FLATTENING Mounting media with microscopic spacers Cell flattening during slide preparation isn’t solely due to hardening mounting media. The capillary pressure between the microscope slide and the cover glass also contributes to this effect. High viscosity mounting medium penetrates biological structures slower and cover glass + slide initiate capillary pressure. This
Mounting spacers: ANTI – FLATTENING Read More »
Mounting is a critical step in microscopy preparation procedures, aimed at preserving the sample and enhancing the image quality during observation. Proper mounting maintains the structural integrity of your specimen, reduces image distortions (like spherical aberration) caused by refractive index mismatch, and allows long-term storage. The protocol The protocol outlined below describes a common mounting
09 Mounting procedure Read More »
DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to DNA. It is commonly used in fluorescence microscopy for visualizing cell nuclei because its fluorescence is enhanced when bound to double-strand DNA. The protocol: For staining of nuclear DNA in common adherent cells, a DAPI concentration in the range of 1-10 µg/mL is usually sufficient.
Secondary antibody staining of the primary antibody. Amplifies the signal and allowing the spatial location of the antibody to be visualized.
07 Secondary antibody staining Read More »
Primary antibody staining is a key step in microscopy immunostaining protocols for specific proteins or other molecules visualization.
06 Primary antibody staining Read More »
Blocking is a crucial step in fluorescence microscopy to prevent nonspecific binding of antibodies and reduce the occurrence of artifacts.
The procedure for transferring the cover glass from the petri dish to a drop of PBS on a Parafilm surface involves several careful steps.
04 From Petri dish to Parafilm Read More »
Triton X-100 permeabilization is used for increasing the biological structures accessibility for antibodies or dyes, to visualize structures.
03 Permeabilization Read More »
The purpose of fixation is to crosslink and maintain cellular components such as proteins, DNA, and RNA in their native state and location.
